Fig. 8

Emodin regulated human PASMC viability and apoptosis under hypoxia exposure by upregulating miR-244-5p. a CCK-8 assay was performed to determine human PASMC viability. b Typical western blotting and quantitative analysis for Ki-67 protein normalized to β-actin. c The Mitochondrial Membrane Potential and Apoptosis Detection Kit with Mito-Tracker Red CMXRos and Annexin V-FITC assay was performed to determine apoptosis of human PASMCs; scale bar = 100 μm. d Typical western blotting and quantitative analysis of cleaved caspase-3 normalized to β-actin. N = 3. *P < 0.05 and **P < 0.01 versus hypoxia + NC inhibitor group; ^#P < 0.05 and ^##P < 0.01 versus hypoxia + emodin + NC inhibitor group