Fig. 3

miR-133a treatment enhanced the sensitivity of A549/DDP cell to DDP. a qRT-PCR was conducted in mimics NC and miR-133a mimics transfected A549/DDP cells to examine the overexpression efficiency of miR-133a. b After transfected with mimics NC and miR-133a mimics for 48 h, the cell viability of A549/DDP cell was examined in the presence of DDP (1 μg/ml) by CCK-8. c The cell proliferation of mimics NC and miR-133a mimics treated A549/DDP cell was detected by colony formation experiment in the presence of DDP (1 μg/ml). d and e After 48 h of mimics NC and miR-133a mimics transfection, the A549/DDP cell was treated with DDP (1 μg/ml) and then subjected for cell cycle and apoptosis detection using flow cytometry analysis. f Protein expression levels of Bcl-2, Bax and cleaved-caspase-3 were measured by western blot in miR-133a mimics transfected A549/DDP cells treated with DDP (1 μg/ml)